Biochemistry Laboratory II, CHE 453L

Monday and Wednesday 1:00-3:50 PM, NSM C-339

Instructor: Dr. Noel Sturm, NSM D-323, nsturm@csudh.edu

Office Hours: TBA.

Prerequisites: (a) CHE 451L and (b) concurrent enrollment in CHE 452

Laboratory Manual: Print, place in a binder and bring to the laboratory:

Experimental Protocol Glucose-1-Phosphate

Experimental Protocol Wheat Germ Acid Phosphatase Purification

Experimental Protocol Wheat Germ Acid Phosphatase Kinetics

Introductory Material

G-1-P Assays

Wheat Germ Assays

Laboratory Reports: 
One lab report for each experiment is required and will be graded for content, discussion of theory and instrument components, quality of data, correctness of calculations, closeness of experimental results to literature values, justifications for poor results, discussion of error, and as outlined in the scoring rubric.

Potato Scoring Rubric

Wheat Germ Scoring Rubric

Written Quiz: Will cover material from both of the laboratories (100 pts). Types of material to emphasize: What reaction does wheat germ acid phosphatase catalyze? What does 7-min hydrolysis followed by the Fiske-Subbarow assay tell us? What do the results of the Biuret assay tell you in the wheat germ lab? vs the results of the phosphatase assay? What is phenyl mercuric nitrate used for in the potato lab?

Writing Assignment: A paper word-processed, double-spaced, 250 words or less, addressing the following tasks is due on Wednesday, May 13th, 2015 (50 pts).

Unexpected challenges in our lives sometimes bring out the best in us. For example, we act heroically or courageously in emergencies, we are charitable when disasters affect our friends, or we are unselfish and patient when misfortune strikes our friends or relatives. Pick an instance in your own life when such a challenge stimulated in you a response that pleased you. Describe the situation, explain how you responded, and tell what you learned about yourself from the experience.

This paper will be judged on rhetoric, how effectively you address the writing tasks and syntax, grammar.

Grading Criteria: Letter grades are based on the following point distribution. Grades are criterion-referenced not norm-referenced; each student has an equal opportunity meet the criteria charted below:

Written Quiz=100 pts

Two Lab Reports@100 pts=200 pts

Writing Assignment=50 pts

Grade Percentage Grade Percentage
A 100-91 A- 90-89
B+ 88-87 B 86-81
B- 80-79 C+ 78-77
C 76-71 C- 70-69
D+ 68-67 D 66-60
F 59-0    

 

Check-In / Introduction W 1/28
Glucose-1-Phosphate / Wheat Germ Discussion    

Group I: Glucose-1-Phosphate, Isolation

Group II: Wheat Germ, Isolation

M 2/2

Group I: Glucose-1-Phosphate, Isolation

Group II: Wheat Germ, Isolation

W 2/4

Group I: Glucose-1-Phosphate, Isolation

Group II: Wheat Germ, Kinetics

M 2/9

Group I: Glucose-1-Phosphate, Isolation

Group II: Wheat Germ, Kinetics

W 2/11
HOLIDAY M 2/16

Group I: Glucose-1-Phosphate, Isolation

Group II: Wheat Germ, Kinetics

W 2/18

Group I: Glucose-1-Phosphate, Isolation

Group II: Wheat Germ, Kinetics

M 2/23

Group I: Glucose-1-Phosphate, Isolation

Group II: Wheat Germ, Kinetics

W 2/25

Group I: Glucose-1-Phosphate, Characterization

Group II: Wheat Germ, Kinetics

M 3/2

Group I: Glucose-1-Phosphate, Characterization

Group II: Wheat Germ, Kinetics

W 3/4

Group I: Glucose-1-Phosphate, Characterization

Group II: Wheat Germ, Kinetics

M 3/9

Group I: Utility Day (Finish G-1-P)

Group II: Utility Day (Finish Wheat Germ)

W 3/11

Group I: Wheat Germ, Isolation (Lab Report Due, 100 Pts)

Group II: Glucose-1-Phosphate, Isolation

M 3/16

Group I: Wheat Germ, Isolation

Group II: Glucose-1-Phosphate, Isolation

W 3/18

Group I: Wheat Germ, Isolation

Group II: Glucose-1-Phosphate, Isolation

M 3/23

Group I: Wheat Germ, Isolation

Group II: Glucose-1-Phosphate, Isolation

W 3/25
HOLIDAY M 3/30
HOLIDAY W 4/1

Group I: Wheat Germ, Kinetics

Group II: Glucose-1-Phosphate, Isolation

M 4/6

Group I: Wheat Germ, Kinetics

Group II: Glucose-1-Phosphate, Isolation

W 4/8

Group I: Wheat Germ, Kinetics

Group II: Glucose-1-Phosphate, Isolation

M 4/13

Group I: Wheat Germ, Kinetics

Group II: Glucose-1-Phosphate, Isolation

W 4/15

Group I: Wheat Germ, Kinetics

Group II: Glucose-1-Phosphate, Isolation

M 4/20

Group I: Wheat Germ, Kinetics

Group II: Glucose-1-Phosphate, Isolation

W 4/22

Group I: Wheat Germ, Kinetics

Group II: Glucose-1-Phosphate, Characterization

M 4/27

Group I: Wheat Germ, Kinetics

Group II: Glucose-1-Phosphate, Characterization

W 4/29

Group I: Wheat Germ, Kinetics

Group II: Glucose-1-Phosphate, Characterization

M 5/4

QUIZ (100 Pts)/Check-Out

W 5/6
Lab Report Due, 100 Pts; Paper Due, 50 Pts W 5/13

© Dr. Noel Sturm 2015