Work Sheet 9

Enzymes: Catalysis and Kinetics

1. Describe the following terms associated with enzymes: catalysis, substrate, reaction specificity, rate-limiting step, coenzyme and prosthetic group.

2. Define the following six types of enzyme reactions: transferase, hydrolase, oxidoreductase, lyase, ligase and isomerase.

3. Define the following terms related to the energy of enzyme reactions: transition state, energy of activation, exergonic, endergonic and free energy change.

4. Discuss the concepts of substrate affinity, K_m and apparent K_m.

5. Use the Lineweaver-Burke plot to demonstrate V_max, K_m, competitive inhibition and non-competitive inhibition.

© Sturm 2014