TLC Report

CHE 317 "Chromatography"

The Report

TLC

1. Include the regular heading.

2. Explain in your own words what chromatography is and how it works; with emphasis on tlc.

3. In a few sentences state what you did in this experiment; be specific.

4. Make a table showing the R_f values for each component for each adsorbent.

5. Conclusion as to the identity of your analgesic(s). Justify your answer.

6. Discuss the results of your experiment.

7. Answer questions:

a) Two components, A and B, were separated by tlc. When the solvent front had moved 10.0 cm above the level of the original spot, the spot corresponding to A was 3.5 cm and that of B was 4.1 cm above the original spot. What are the retention factors for A and B? Which component would elude first from a column chromatography using the same absorbant and solvent?

b) Describe how thin layer chromatography could be used to analyze a urine sample for the presence of cocaine if you had a known sample in the lab.

c) Why is it important that the sample to be analyzed by tlc be dry?

d) Why must the solvent level be below the original spot on the tlc plate?

e) Why must the cap be in place on the bottle during the tlc separation?

Attach the carbons from your notebook.